IN VITRO CULTIVATION OF SCHIZONTS OF SARCOCYSTIS SPEERI Dubey and Lindsay, 1999

J. P. Dubey; C. A. Speer; D. S. Lindsay

doi:10.1645/0022-3395(2000)086[0671:IVCOSO]2.0.CO;2

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J. P. Dubey,¹ C. A. Speer,^1,* D. S. Lindsay^1,**

¹United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Scienc

^**Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717-3610.
^**†Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia–Maryland Regional College of Veterinary Medicine, Virginia Tech, 1410 Prices Road, Blacksburg, Virginia 240061-0342.

Abstract

Schizonts of Sarcocystis speeri Dubey and Lindsay, 1999 were cultured in vitro in bovine monocyte and equine kidney cell cultures inoculated with infected tissues of nude and γ-interferon knockout mice fed sporocysts from opossums, Didelphis albiventris. At least 1 asexual cycle was completed in 3 days. In vitro-grown merozoites were structurally and antigenically distinct from those of Sarcocystis neurona and Sarcocystis falcatula. Culture-derived merozoites of S. speeri were not infective to budgerigars (Melopsittacus undulatus).

Citation Download Citation

J. P. Dubey, C. A. Speer, and D. S. Lindsay "IN VITRO CULTIVATION OF SCHIZONTS OF SARCOCYSTIS SPEERI," Journal of Parasitology 86(4), 671-678, (1 August 2000). https://doi.org/10.1645/0022-3395(2000)086[0671:IVCOSO]2.0.CO;2

Received: 3 August 1999; Accepted: 1 January 2000; Published: 1 August 2000

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